HiTrap SP HP column - 5 × 1 mL

HiTrap SP HP is a prepacked column designed for high-resolution, small-scale purification of proteins using strong cation exchange chromatography. Packed with SP Sepharose™ High Performance resin featuring a 34 µm particle size, this column delivers exceptional resolution and efficient separation across a broad pH range.

Key Features

✅ Strong Cation Exchange Resin
Utilizes SP Sepharose HP for strong cation exchange, ideal for purifying positively charged proteins.

✅ High Resolution and Performance
Small 34 µm bead size provides sharper peaks and higher binding capacity for complex protein samples.

✅ Flexible Application
Operates with a syringe, peristaltic pump, or chromatography systems such as ÄKTA™ FPLC—suitable for stand-alone use or in series.

✅ Wide pH Compatibility
Works effectively across a broad pH range, offering flexibility in method development and protein optimization.

Proven Research Applications

HiTrap SP HP columns have been successfully used in peer-reviewed studies, including:

• Recombinant Histone Purification from cell extracts for epigenetic and structural studies
• Purification of BpsOMP38 to research porin involvement in cephalosporin and carbapenem resistance of Burkholderia pseudomallei
  
• Hematopoietic Stem Cell Expansion, utilizing recombinant HOXB4 in FPLC workflows