ELISA Phospho-RAF1 (S621) anti-

Quantity :50µL Clone Number:1C2 Aliases:RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF Product Type:Recombinant Antibody Immunogen Species: UniProt ID:P04049 Immunogen:A syntheQuantityd peptide derived from Phospho-RAF1 (S621) Raised in: Reactivity: Tested Applications:ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 Background:Serine/threonine-protein kinase that acts as a regµLatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regµLatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellµLar signal-regµLated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resµLting in their activation. Phosphorylates PPP1R12A resµLting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. RegµLates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regµLation of a transcriptional repressor SNAI2/SLµg, which induces down-regµLation of OCLN. Restricts caspase activation in response to selected stimµLi, notably Fas stimµLation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Clonality:Monoclonal Isotype:Rabbit IgG Purification Method:Affinity-chromatography Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:RAF1 Research Areas:Signal Transduction