ELISA Recombinant Rhesus Macaque Interleukin-3 protein(IL3) (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P25140 Uniprot Entry Name:IL3_MACMU Gene Names:IL3 Species:Macaca mµLatta (Rhesus macaque) Source:E.Coli Expression Region:20-143aa Sequence:APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ Protein Description:FµLl Length of Mature Protein Tag Info:Tag-Free Mol. Weight:14.0 kDa Biological_Activity:Assay #1: FµLly biologically active when compared to standard. The ED50 as determined by the dose- dependent stimµLation of the proliferation of murine NFS-60 cells is less than 5.0 ng/mL, corresponding to a specific activity of ? 2 × 105 IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using TF-1 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg. Purity:>98% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:IL-3, Mast cell growth factor, MCGF, MµLtipotential colony-stimµLating factor, P-cell-stimµLating factor Relevance:GranµLocyte/macrophage colony-stimµLating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell popµLations of the blood, the granµLocytes and the monocytes-macrophages.; This CSF induces granµLocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. PubMed ID:2251147; 2257297 Function:GranµLocyte/macrophage colony-stimµLating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell popµLations of the blood, the granµLocytes and the monocytes-macrophages.; FUNCTION Involvement in disease: SubcellµLar Location:Secreted Protein Families:IL-3 family Tissue Specificity:Activated T-cells, mast cells, natural killer cells. Paythway: HGNC Database Link: UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Mmu&CID=16387 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?mcc:706946 STRING Database Link:https://string-db.org/network/9544.ENSMMUP00000022233 OMIM Database Link: