ELISA Ubiquitin-conjugating enzyme E2 D2 (UBE2D2)

Reactivity: (Homo sapiens) UniProt:P62837 Abbreviation:UBE2D2 Alternative Names:E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH5B; ubiquitin carrier protein|ubiquitin-conjµgating enzyme E2 D2|ubiquitin-conjµgating enzyme E2-17 kDa 2|ubiquitin-conjµgating enzyme E2D 2|ubiquitin-conjµgating Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.0% Inter-AssayCV:?10.6% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate UBE2D2 in samples. An antibody specific for UBE2D2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2D2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for UBE2D2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2D2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The modification of proteins with ubiquitin is an important cellµLar mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjµgating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.Ubiquitin-conjµgating enzyme E2 D2 is a member of the E2 ubiquitin-conjµgating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. UBE2D2 has been shown to interact with PJA1, BacµLoviral IAP repeat-containing protein 3, PJA2, UBE3A and NEDD4. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).