ELISA Ubiquitin-60S ribosomal protein L40 (UBA52)

Reactivity: (Homo sapiens) UniProt:P63048 Abbreviation:UBA52 Alternative Names:CEP52; HUBCEP52; MGC126879; MGC126881; MGC57125; RPL40; 60S ribosomal protein L40|ribosomal protein L40|ubiquitin and ribosomal protein L40|ubiquitin carboxyl extension protein 52|ubiquitin-52 amino Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.116 ng/mL Intra-AssayCV:?4.2% Inter-AssayCV:?8.1% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate UBA52 in samples. An antibody specific for UBA52 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBA52 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for UBA52 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBA52 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellµLar proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regµLation of gene expression, and the stress response. Ubiquitin is syntheQuantityd as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). MµLtiple processed pseudogenes derived from this gene are present in the genome. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).