ELISA 2-acylglycerol O-acyltransferase 2 (MOGAT2)

Reactivity: (Homo sapiens) UniProt:Q3SYC2 Abbreviation:MOGAT2 Alternative Names:DGAT2L5; FLJ22644; MGAT2; MGC119183; MGC119184; MGC119185; acyl CoA:monoacylglycerol acyltransferase 2 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.056 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.5% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MOGAT2 in samples. An antibody specific for MOGAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOGAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MOGAT2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOGAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:MOGAT2 contains a phosphate acyltransferase-like domain and 2 predicted N-linked glycosylation sites. Northern blot analysis revealed expression of 7.2-, 3.0-, and 1.3-kb transcripts in liver, stomach, and small intestine, with lower expression in colon and kidney. A probe specific to the truncated splice variant hybridized mainly to the 1.3-kb transcript in stomach. Immunofluorescence analysis of transiently transfected COS-7 cells showed that MGAT2 displayed a pattern consistent with localization in the endoplasmic reticµLum.Substrate specificity assays of MOGAT2 demonstrated that MOGAT2 can catalyze the acylation of rac-1- and sn-2-monooleoylglycerols. MOGAT2 uses a broad range of fatty acyl-CoAs but prefers monoacylglycerols containing unsaturated fatty acids. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).