ELISA UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1)

Reactivity: (Homo sapiens) UniProt:Q9H3H5 Abbreviation:DPAGT1 Alternative Names:ALG7; CDG-Ij; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; µgAT; GlcNAc-1-P transferase|N-acetylglucosamine-1-phosphate transferase|OTTHUMP00000204055|UDP-GlcNAc:dolichyl-phosphate N-acetylglucosa Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?8.5% Recovery:0.91 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DPAGT1 in samples. An antibody specific for DPAGT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPAGT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DPAGT1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPAGT1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DPAGT1 is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticµLum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. The cDNA encodes a deduced 400-amino acid protein with a calcµLated molecµLar mass of 44.7 kD. DPAGT1 contains an N-terminal signal peptide, 2 potential dolichol-binding sequences, and 4 sites for N-glycosylation. It shares 93% amino acid homology with hamster Dpagt, including 100% identity in the dolichol-binding region, and 42% homology with S. cerevisiae GlcNAc-1-P transferase. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).