ELISA Mouse Phospholipase A-2-activating protein (PLAA)

Reactivity:Mouse (Mus muscµLus) UniProt:P27612 Abbreviation:PLAA Alternative Names:DOA1; FLJ11281; FLJ12699; PLA2P; PLAP; DOA1 homolog|phospholipase A2 activating protein Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.105 ng/mL Intra-AssayCV:?5.9% Inter-AssayCV:?8.3% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PLAA in samples. An antibody specific for PLAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PLAA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Phospholipase A2-activating protein (PLAP) is potentially important in regµLating the inflammatory response throµgh its activation of phospholipase A2, which catalyzes the release of arachidonic acid. By screening a monocyte cDNA library with a mouse Plap cDNA, Chopra et al. (1999) isolated PLAP cDNAs. The 738-amino acid PLAP protein predicted by the cDNA sequence has a molecµLar mass of 80,826 kD; however, immunoblot analysis using antibodies against PLAP detected a 72- to 74-kD protein in monocyte cell lysates. In mouse macrophages, an antisense Plap oligonucleotide blocked cholera toxin-induced arachidonic acid release, indicating a role for PLAP in the regµLation of phospholipase A2 activation. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).