ELISA Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA)

Reactivity: (Homo sapiens) UniProt:Q9BZF9 Abbreviation:UACA Alternative Names:FLJ10128; KIAA1561; MGC141967; MGC141969; NUCLING; nuclear membrane binding protein|uveal autoantigen with coiled coil domains and ankyrin repeats Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.108 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?8.4% Recovery:0.94 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate UACA in samples. An antibody specific for UACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for UACA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UACA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Using a sequence homologous to the bovine UACA sequence to screen a Jurkat cell cDNA library, they isolated the homolog, which was found have the same sequence as KIAA1561. UACA encodes a deduced 1,449-amino acid protein with a predicted molecµLar mass of approximately 166 kD. It contains 6 ankyrin repeats, a leucine zipper motif, and coiled-coil domains. The bovine and proteins share 86% sequence homology. Northern blot analysis demonstrated expression at various levels in all tissues analyzed, with highest expression in skeletal muscle.the prevalence of IgG anti-UACA autoantibodies in patients with panuveitis was significantly higher than that in healthy controls (19.6-28.1% vs 0%, p less than 0.05), indicating that autoimmunity directed against UACA is a common phenomenon in these diseases. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).