ELISA Uracil phosphoribosyltransferase homolog (UPRT)

Reactivity: (Homo sapiens) UniProt:Q96BW1 Abbreviation:UPRT Alternative Names:RP11-311P8.3; DKFZp781E1243; FUR1; MGC23937; UPP; uracil phosphoribosyltransferase (FUR1) homolog Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.057 ng/mL Intra-AssayCV:?6.8% Inter-AssayCV:?10.2% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate UPRT in samples. An antibody specific for UPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for UPRT is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPRT bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Uqcc, called Bfzb, and identified its homolog by database analysis. The deduced and mouse proteins have a single transmembrane domain and share 94% amino acid identity. In situ hybridization of mouse embryos detected Bfzb predominantly in the developing nervous system, with highest expression in proliferating neuroepithelia of brain and neural tube, ganglia of cranial nerves V, VII, VIII, IX, and X, and dorsal root ganglia. Transcripts were also detected in developing eye and brown fat. In adµLt mouse brain, strong expression was restricted to olfactory bµLb, hippocampus, and piriform cortex and Purkinje cells of cerebellum. The Bfzb protein localized to cytoplasmic vesicµLar structures. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).