ELISA Mouse Thy-1 membrane glycoprotein (THY1)

Reactivity:Mouse (Mus muscµLus) UniProt:P01831 Abbreviation:THY1 Alternative Names:CD90; FLJ33325; Thy-1 T-cell antigen|Thy-1 membrane glycoprotein Application:ELISA Range: 12.50-800 pg/mL Sensitivity:5.7 pg/mL Intra-AssayCV:?5.2% Inter-AssayCV:?7.2% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate THY1 in samples. An antibody specific for THY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTHY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for THY1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of THY1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Thy-1 or CD90 (Cluster of Differentiation 90) is a 25 ndash 37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobµLin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the ImmunoglobµLin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).