ELISA Mouse Tumor necrosis factor receptor superfamily member 3 (LTBR)

Reactivity:Mouse (Mus muscµLus) UniProt:P50284 Abbreviation:LTBR Alternative Names:CD18; D12S370; LT-BETA-R; TNF-R-III; TNFCR; TNFR-RP; TNFR2-RP; TNFRSF3; TNFR superfamily; member 3|lymphotoxin B receptor|lymphotoxin beta receptor|tumor necrosis factor C receptor|tumor necrosis fa Application:ELISA Range:31.25-2000 pg/mL Sensitivity:7.8 pg/mL Intra-AssayCV:?4.4% Inter-AssayCV:?8.7% Recovery:0.95 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LTBR in samples. An antibody specific for LTBR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLTBR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LTBR is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTBR bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:This protein is a member of the tumor necrosis factor (TNF) family of receptors. It is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. The protein specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). The encoded protein and its ligand play a role in the development and organization of lymphoid tissue and tranformed cells. Activation of the encoded protein can trigger apoptosis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).