ELISA 26S proteasome non-ATPase regulatory subunit 10 (PSMD10)

Reactivity: (Homo sapiens) UniProt:O75832 Abbreviation:PSMD10 Alternative Names:RP5-889N15.3; dJ889N15.2; p28; 26S proteasome non-ATPase regµLatory subunit 10|26S proteasome regµLatory subunit p28|ankyrin repeat protein|gankyrin|hepatocellµLar carcinoma-associated protein p28-I Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?4.4% Inter-AssayCV:?8.3% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PSMD10 in samples. An antibody specific for PSMD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PSMD10 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMD10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The 26S proteasome is a mµLticatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regµLator. Proteasomes are distributed throµghout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD10 encodes a non-ATPase subunit of the 19S regµLator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.The predicted 226-amino acid p28 protein contains 5 ankyrin repeats, which are thoµght to function in protein-protein interactions. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).