ELISA Sialoadhesin (SIGLEC1)

Reactivity: (Homo sapiens) UniProt:Q9BZZ2 Abbreviation:SIGLEC1 Alternative Names:CD169; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; SIGLEC-1; SN; dJ1009E24.1; sialic acid-binding immunoglobµLin-like lectin 1|sialoadhesin Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.056 ng/mL Intra-AssayCV:?7.4% Inter-AssayCV:?11.1% Recovery:1.08 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SIGLEC1 in samples. An antibody specific for SIGLEC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SIGLEC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Sialoadhesin is a cell adhesion molecµLe found on the surface of certain cells of the immune system called macrophages. It is defined as an I-type lectin, since it contains 17 immunoglobµLin (Ig) domains (one variable domain and 16 constant domains), and thus also belongs to the immunoglobµLin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. Alternate names for sialoadhesin include siglec-1 and CD169 (cluster of differentiation 169). Sialoadhesin predominately binds neutrophils, but can also bind monocytes, natural killer cells, B cells and a subset of cytotoxic T cells by interacting with sialic acid molecµLes in the ligands on their surfaces. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).