ELISA Synaptic vesicular amine transporter (SLC18A2)

Reactivity: (Homo sapiens) UniProt:Q05940 Abbreviation:SLC18A2 Alternative Names:MGC120477; MGC120478; MGC26538; SVAT; SVMT; VAT2; VMAT2; vesicle monoamine transporter type 2 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.055 ng/mL Intra-AssayCV:?6.2% Inter-AssayCV:?9.4% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC18A2 in samples. An antibody specific for SLC18A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC18A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLC18A2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC18A2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:VMAT2 is an integral membrane protein that acts to transport monoamines—particµLarly neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine—from cellµLar cytosol into synaptic vesicles. In s, the VMAT2 protein is encoded by the VMAT2 gene. Geneticist Dean Hamer identified the VMAT2 gene as correlating with spirituality using data from a smoking survey, which included questions intended to measure "self-transcendence". Hamer performed the spirituality study on the side, independently of the National Cancer Institute smoking study. His findings were published in the mass-market book According to Carl Zimmer, Hamer's study has not been academically published and depends on minor statistical variance. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).