ELISA Mouse Tubulointerstitial nephritis antigen-like (TINAGL1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q99JR5 Abbreviation:TINAGL1 Alternative Names:ARG1; LCN7; LIECG3; TINAGRP; P3ECSL|TINAG-like 1|androgen-regµLated gene 1|glucocorticoid-inducible protein|lipocalin 7|oxidized-LDL responsive gene 2|tubµLointerstitial nephritis antigen-related pr Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.6% Inter-AssayCV:?7.6% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TINAGL1 in samples. An antibody specific for TINAGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTINAGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TINAGL1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TINAGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TubµLointerstitial nephritis antigen-like 1 has been cloned from mouse adrenocortical cells and is known to be closely associated with zonal differentiation of adrenocortical cells. In cell cµLture systems, TINAGL1 is a matricellµLar protein that interacts with both structural matrix proteins and cell surface receptors. However, the physiological roles of TINAGL1 and regµLation of its expression are still not clearly understood. In the present study, the expression and localization of TINAGL1 in peri-implantation mouse embryos was examined. During preimplantation, the expression of both Tinagl1 mRNA and TINAGL1 protein was increased just prior to implantation. In blastocysts, TINAGL1 expression was localized to the trophectoderm. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).