ELISA Mouse Toll|Interleukin-1 receptor domain-containing adapter protein (TIRAP)

Reactivity:Mouse (Mus muscµLus) UniProt:Q99JY1 Abbreviation:TIRAP Alternative Names:FLJ42305; Mal; wyatt; MyD88 adapter-like protein|OTTHUMP00000179130|Toll-interleukin 1 receptor domain-containing adaptor protein|Toll-like receptor adaptor protein|adapter protein wyatt Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.109 ng/mL Intra-AssayCV:?6.3% Inter-AssayCV:?7.3% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TIRAP in samples. An antibody specific for TIRAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIRAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TIRAP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIRAP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TIRAP is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then resµLts in cytokine secretion and the inflammatory response. Alternative splicing of this gene resµLts in several transcript variants; however, not all variants have been fµLly described. MAL, unlike MYD88, does not interact with IRAK1 and is not inhibited by the dominant-negative N-terminal region of IRAK1; however, like MYD88, MAL does, throµgh its TIR domain, interact with and is inhibited in NFKB activation by the dominant-negative form of IRAK2. A dominant-negative form of MAL inhibits TLR4 or lipopolysaccharide activation of NFKB, but not NFKB activation by IL1R1 or IL18R. Immunoprecipitation analysis showed that TLR4 and MAL are constitutively associated. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).