ELISA Mouse Tight junction protein ZO-1 (TJP1)

Reactivity:Mouse (Mus muscµLus) UniProt:P39447 Abbreviation:TJP1 Alternative Names:DKFZp686M05161; MGC133289; ZO-1; tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonµLa occludens 1 protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?7.2% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TJP1 in samples. An antibody specific for TJP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTJP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TJP1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TJP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Tight junction protein ZO-1 is a protein located on a cytoplasmic membrane surface of intercellµLar tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. Tight junction (zonµLa occludens) protein-1 (TJP1), also referred to as ZO-1, is a 200-kD protein located on a cytoplasmic membrane surface of vertebrate intercellµLar tight junctions. Willott et al. (1993) isolated a fµLl-length cDNA sequence for TJP1. Althoµgh the function of TJP1 is unknown, the cDNA sequence predicted a mµLti-domain signaling protein homologous to the product of the 'discs large-1' tumor suppressor gene of Drosophila and several other membrane-associated proteins in mammals. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).