ELISA Mouse Transketolase-like protein 1 (TKTL1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q99MX0 Abbreviation:TKTL1 Alternative Names:LL0XNC01-14B7.1; TKR; TKT2; transketolase-2|transketolase-related protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.063 ng/mL Intra-AssayCV:?6.1% Inter-AssayCV:?8.4% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TKTL1 in samples. An antibody specific for TKTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTKTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TKTL1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TKTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Transketolase (TKT;) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. As part of a systematic search for differentially expressed genes, Coy et al. (1996) isolated a novel transketolase-related gene, TKTL1, which they called TKR. They isolated transcripts encoding tissue-specific protein isoforms. Comparison with known transketolases demonstrated a TKR-specific deletion mutating 1 thiamine-binding site. Genomic sequencing of the TKR gene by Coy et al. (1996) revealed the presence of a pseudoexon, as well as the acquisition of a tissue-specific spliced exon, compared to the TKT gene, which maps to 3p14.3. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).