ELISA Mouse Toll-like receptor 5 (TLR-5)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9JLF7 Abbreviation:TLR5 Alternative Names:RP11-239E10.1; FLJ10052; MGC126430; MGC126431; SLEB1; TIL3; OTTHUMP00000036039|toll/interleukin-1 receptor-like protein 3 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.064 ng/mL Intra-AssayCV:?4.5% Inter-AssayCV:?6.5% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TLR5 in samples. An antibody specific for TLR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TLR5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Toll-like receptor 5is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to s and share structural and functional similarities. They recognize pathogen-associated molecµLar patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene product is expressed in myelomonocytic cells, and recognizes bacterial flagellin, a principal component of bacterial flagella and a virµLence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB and stimµLates tumour necrosis factor-alpha production. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).