ELISA Mouse Transmembrane protease serine 4 (TMPRSS4)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8VCA5 Abbreviation:TMPRSS4 Alternative Names:MT-SP2; TMPRSS3; membrane-type serine protease 2|transmembrane serine protease 3|type II membrane serine protease Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.063 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?10.1% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMPRSS4 in samples. An antibody specific for TMPRSS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMPRSS4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TMPRSS4 encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellµLar region containing the serine protease domain. MµLtiple transcript variants encoding different isoforms have been found for this gene.A weak TMPRSS4 signal was detected in normal tissues of the gastrointestinal tract and in some of the urogenital tract but was not expressed in any other normal tissues. The authors sµggested that TMPRSS4 may be important in processes involved in metastasis formation and tumor invasion. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).