ELISA Mouse Thioredoxin-related transmembrane protein 1 (TMX1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8VBT0 Abbreviation:TMX1 Alternative Names:DKFZp564E1962; PDIA11; TMX; TXNDC; TXNDC1; protein disµLfide isomerase family A; member 11|thioredoxin domain containing 1|thioredoxin domain-containing Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?3.9% Inter-AssayCV:?6.7% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMX1 in samples. An antibody specific for TMX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMX1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TXNDC1 is a thioredoxin (TXN)-related protein with disµLfide reductase activity. The deduced 280-amino acid protein has a predicted molecµLar mass of 31.8 kD and contains an N-terminal signal sequence, a TRX-like domain with an active site sequence CPAC, and a transmembrane domain. Northern blot analysis detected a 2.5-kb transcript in all tissues examined, with highest expression in kidney, liver, placenta, and lung. Confocal microscopy of intact HEK293 cells expressing TXNDC1 and Western analysis of subcellµLar fractions localized TXNDC1 primarily in the microsomal fraction in an endoplasmic reticµLum (ER)-like staining pattern characterized by a diffuse network-like labeling of the cytoplasm and nuclear rim. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).