ELISA Mouse Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9CR75 Abbreviation:TNFRSF12A Alternative Names:CD266; FN14; TWEAKR; type I transmembrane protein Fn14 Application:ELISA Range:125-2000 pg/mL Sensitivity:50 pg/mL Intra-AssayCV:?4.4% Inter-AssayCV:?8.1% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNFRSF12A in samples. An antibody specific for TNFRSF12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNFRSF12A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF12A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:FN14 protein, which shares 82% amino acid identity with the mouse sequence, contains a signal peptide, an extracellµLar domain, a membrane-anchoring domain, and a cytoplasmic domain. Northern blot analysis detected increased FN14 expression in response to FGF1, calf serum, or phorbol ester stimµLation of quiescent fibroblasts in vitro. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated FN14 expression was found in liver cancer cell lines and hepatocellµLar carcinoma specimens. Expression of mouse Fn14 was upregµLated in hepatocellµLar carcinoma nodµLes that develop in 2 different transgenic mouse models of hepatocarcinogenesis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).