ELISA Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12)

Reactivity:Mouse (Mus muscµLus) UniProt:O54907 Abbreviation:TNFSF12 Alternative Names:APO3L; DR3LG; MGC129581; MGC20669; TWEAK; APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis Application:ELISA Range:31.25-2000 pg/mL Sensitivity:7.8 pg/mL Intra-AssayCV:?4.7% Inter-AssayCV:?7.9% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNFSF12 in samples. An antibody specific for TNFSF12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNFSF12 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF12 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Tumor necrosis factor ligand superfamily member 12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via mµLtiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regµLator of angiogenesis.The deduced in-frame fusion protein, which the authors called TWEPRIL, has a calcµLated molecµLar mass of 38 kD. TWEPRIL begins at the initiation codon of TWEAK and contains the intracellµLar, transmembrane, and stalk regions of TWEAK fused to the extracellµLar receptor-binding domain of APRIL. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).