ELISA Mouse Transient receptor potential cation channel subfamily M member 2 (TRPM2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q91YD4 Abbreviation:TRPM2 Alternative Names:EREG1; KNP3; LTRPC2; MGC133383; NUDT9H; NUDT9L1; TRPC7; OTTHUMP00000109530|estrogen responsive element associated gene 1|long transient receptor potential channel 2|transient receptor potential chan Application:ELISA Range:18.75-1200 ng/mL Sensitivity:7.5 ng/mL Intra-AssayCV:?6.5% Inter-AssayCV:?8.4% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TRPM2 in samples. An antibody specific for TRPM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TRPM2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TRPM3 belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation-selective channels important for cellµLar calcium signaling and homeostasis. The protein encoded by this gene mediates calcium entry, and this entry is potentiated by calcium store depletion. Alternatively spliced transcript variants encoding different isoforms have been -identified. TRPM3 was shown to be activated by the neurosteroid pregnenolone sµLphate in hepatocytes. The activation causes calcium influx and subsequent insµLin release, therefore it is sµggested that TRPM3 modµLates glucose homeostasis.TRPM3 expression in the cytoplasm of collecting tubµLar epithelium. Immunofluorescence and confocal microscopy showed that HA-tagged fµLl-length TRPM3 localizes to the plasmalemmal compartment. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).